• Hydrolysates are proteins digested into smaller fragments, peptides, and its sole building blocks, the amino acids. A hydrolysate is therefore a blend of different peptides and different peptide lengths. In the hydrolysis process, we start with the intact protein, for instance whey or casein, which is digested by enzymes into smaller fragments. The hydrolysis process influences the peptide size. Depending on the level of hydrolysis, we are able to obtain mildly hydrolysed, partially hydrolysed or extensively hydrolysed proteins. Moreover, the peptides can be filtered, in order to obtain a mixture with very small peptides and no larger fragments.
    The degree of hydrolysis is defined as the percentage of the number of peptide bonds in a protein which have been cleaved during hydrolysis. Upon enzymatic hydrolysis of the peptide bonds in a protein, new amino groups are formed, one amino group for each broken peptide bond. The amount of newly formed amino groups causes a linear increase in amino nitrogen. This amino nitrogen can be determined by formol titration. In the calculation of the degree of hydrolysis, the increase of amino nitrogen, corrected for the total number of peptide bonds available for cleavage in the protein is calculated.
    Hydrolysates are hydroscopic and need to be kept dry.  Therefore the optimum storage to prolong their life is tightly sealed, air tight containers or bags. These containers or bags should only be opened when they are ready to be used.
    • In our Hydrolysates knowledge center we explain thoroughly, the hydrolysis process, as well as various product characteristics and how they are measured.
    • Additional recommended reading for each specific market segment can be found in the Science library.
    • You can also contact us for information.
    We produce protein hydrolysates for Infant Nutrition, Medical Nutrition and Cell Nutrition.
    • The infant nutrition segment includes protein hydrolysates for hypo-allergenic formulas and easy-to-digest formulas.
    • Cell Nutrition comprises of hydrolysates for Biopharma and Microbial Nutrition applications.
    • FrieslandCampina Domo produces hydrolysates for Sports Nutrition which are marketed by FrieslandCampina DMV.
    For the Infant, Medical and Sports nutrition market we have developed Hyvital® protein hydrolysates. We also produce a range of high quality non-animal hydrolysates for the bioharmaceutical industry and a wide product range of peptones for the microbial nutrition market under the brand names Proyield® and Stedygro®.
    Yes.  The product data sheets are downloadable through the detailed views of the individual products. The detailed views can be visited through each specific market overviews or the product finder. The product data sheets address product characteristics, recommended application fields and typical data for a specific product. For guaranteed specifications (product specification sheets) please contact us.
    If you cannot find the product or information you are looking for then please contact us.
    • We can develop new hydrolysates or customize an existing protein hydrolysate to precisely meet your needs
    • We can provide application support, showing you, for example, how our hydrolysates can be compatible with your product and processing
    • We can supply you with technical information in addition to the information available on this website
    The information on this website is intended for industrial customers only and not intended for consumers. Furthermore, potential consumer benefits related to our products are not to be considered as health claims. They should be considered as potential leads that might be developed into health claims complying with the local legal requirements.
  • Depending on the base medium used for the hydrolysates, the ultrafiltered hydrolysates have a 10 – 20% solubility.  Proyield® Pea PCE80B is the exception having an approximate 5% solubility.
    Standard practice to determine a hydrolysate concentration in a medium formulation, is to titrate the hydrolysate, usually between a  1-8 gm/L concentration.  This titration should be performed using the base medium of choice.
    Selecting a base medium should be performed in concert with selecting your hydrolysate. The chemicals in the base medium can sometimes have a profound effect upon the overall outcome of the cell culture process.
    Our range of protein hydrolysates for Biopharma can be found here:
  • Most of these hydrolysates are soluble at 5% solutions, some will be soluble at up to 10%.
    Our range of protein hydrolysates for microbial nutrition can be found here:
    Yes, however consideration is needed regarding the overall nutritional composition of the medium and the purpose of the fermentation. In many cases, it is possible to improve the yield of the medium.
  • We offer protein hydrolysates containing peptide-bonded glutamine. Glutamine is the single most abundant amino acid in the body being conditionally limited upon strenuous conditions like illness. We offer a range of hydrolysates derived from various protein sources to meet the diverse needs of medical nutrition formulators. Please contact us to discuss solutions for your application.
    Hyvital® Wheat Glutamine hydrolysates are derived from natural wheat protein. Using an enzymatic treatment, the large protein molecules are split into small groups of amino acids, called peptides, which contain the amino acid Glutamine. The enzymes used are food grade, there are two variants: Hyvital® Wheat Glutamine GN and Hyvital® Wheat Glutamine PA. The latter is specifically designed for acidic formulations.
    Hyvital Wheat Glutamine contains peptide-bonded glutamine. Numerous clinical investigations have shown that glutamine enriched medical nutrition support decreases hospital-acquired infections and improves other clinical outcomes (hospital length of stay, post-hospital survival) in critically ill patients1-3
    Hyvital® Wheat Glutamine contains Peptide-bonded glutamine, which is a natural source of glutamine in comparison to free glutamine which is a synthetically prepared amino acid. Furthermore, peptide-bonded glutamine comes with application advantages:
    • It is stable in liquid drinks: Glutamine as a free amino acid is unstable, and may even form toxic substances upon heating.
    • It is heat stable. Free Glutamine may undergo ring condensation to form pyroglutamic acid which is suspected to have neurotoxic properties.
    Our range of protein hydrolysates for medical nutrition can be found here:
  • Protein hydrolysates for Sports Nutrition (or Performance and Life Style Nutrition) are marketed by FrieslandCampina DMV. On this website you can find a brief overview of the product range of FrieslandCampina DMV.